The hCMEC/D3 cells (passage 2832) were seeded on collagen coated culture flasks (2.5-3 10 4 /cm 2 ) or glass slides (4 10 4 /cm 2 ) and maintained at 37C with 5% CO 2 exposure in EBM-2 basal medium (Lonza, Walkersville, MD, USA) supplemented with 5% FBS (Atlanta Biologicals, Lawrenceville, GA, USA), chemically defined lipid concentrate (Life Technologies, Carlsbad, CA, USA), growth factors, antibiotic/antimycotic (1:1, Atlanta Biologicals, GA, USA and HEPES (10 mM)
To back up a little bit, Kraemer has already paid financial penalties for his bad habit and likely ruffled the feathers of the anti-smoking authorities
FAQs Can I vape or use nicotine patches instead of smoking
Putting the disputed conclusions of this particular study aside, there are some legitimate concerns that should be investigated by anyone considering smoked marijuana as their preferred method of cannabis therapy